VOPROSY MEDITSINSKOI KHIMII (ISSN 0042-8809)

Transient minor fractions of human serum albumin: physico-chemical properties

   
Azhitskiĭ G.Yu., Troitskiĭ G.B., Sharaeva T.K., Selivanova K.F., Sokolova N.N.
PubMed Id: 6528520
Year: 1984  Volume: 30  Issue: 5  Pages: 50-55
From 7 to 8 minor fractions were isolated from normal and pathologically altered human blood serum albumin by means of isoelectric focusing in borate-polyol system. As distinct from the whole albumin molecule, these minor fractions contained 12-14% carbohydrates, I-1.2% of which were linked by covalent bonds while the rest of it were absorbed. The fractions included a number of amides, amount of which was gradually elevated with an increase in the isoelectric point. As shown by circular dichroism studies content of alpha-spirals decreased from 20% to 5% in the proteins, depending on the pI value. Spontaneous deamidation was noted during storage. Normally the content of these proteins constituted from 3% to 4%; in liver cirrhosis it might increase up to 50%.
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Azhitskiĭ G.Yu., Troitskiĭ G.B., Sharaeva T.K., Selivanova K.F., Sokolova N.N. (1984) Voprosy meditsinskoi khimii, 30(5), 50-55.
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